All of them.
But in my headcanon Zoro isn't because it was hinted at something going on between him and perona in the anime and in the perona novel.
Nami is a virgin.
Usopp is a virgin.
Sanji lost his virginity in okama island just not the dick virginity.
Chopper is a virgin
Robin had to do some stuff to survive if you catch my meaning
all the older SHs are not virgins.
Usopp and Chopper are the only sure virgins, the rest got laid at least once
Luffy could be virgin too but iirc was implied that Hancock fucked him during the TS in some SBS
That would be Luffy best feat no cap
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