Worstgen

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Oda actually took effort to make Kiku appear trans.

He made her effeminate, both in appearance and dressing.

He made other characters treat her as a woman.
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In contrast, he did nothing of the sort for Yamato.

He made her another oversexualised Nami clone.

He shows off her tiddies at any opportunity.
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He pulled a cheap pervert gag with Momonosuke and Yamato.
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He had Luffy react with disbelief when Yamato introduced themselves as "Kaido's son".

He had Ace's crew call her Kaido's daughter.
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Had Oda been intending to depict Yamato as male, he could have them actually place effort in modifying their appearance to appear less feminine.
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They could use breast bindings instead of wearing clothing that shows off their tiddies.

Oda could have introduced them as Kaido's son instead of his daughter.
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Oda could have had Yamato correct Ace's crew for addressing them as Kaido's son if that was indeed misgendering them.
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