So far out of the 4 members of mads we know queen is the only one to not make use of lineage factor related science
- Judge was able to modify his children along with create clones
- Caesar created sad using it the key ingredient to smile fruits
- Vegapunk was able to create momonosuke's fruit
- Also feeding devil fruits to inanimate objects is probably due to manipulating lineage from. Maybe the rumble ball and whatever drug black maria took